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. 2006 Oct 19;97(12):1417–1423. doi: 10.1111/j.1349-7006.2006.00340.x

Figure 5.

Figure 5

Effect of AG490 on STAT3 activation. (A) Protein samples extracted from SW1990 cells treated for 48 h with AG490 were subjected to western blotting for p‐STAT3 and β‐actin proteins. AG490 significantly decreased p‐STAT3 protein expression in SW1990 cells in a dose‐dependent manner after 48 h incubation. The levels of β‐actin expression were determined as a control for equivalent protein loading. (B) Nuclear protein samples extracted from SW1990 cells after the treatments with AG490 for 48 h were subjected to EMSA for STAT3 DNA‐binding activity. AG490 significantly inhibited STAT3 DNA‐binding activity in a dose‐dependent manner in SW1990 cells after 48 h incubation.