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. 2006 Nov 3;98(1):77–82. doi: 10.1111/j.1349-7006.2006.00355.x

Figure 3.

Figure 3

Localization of S100A10 and annexin II complex on the membrane of cultured renal cell carcinoma cells. (A) Pull‐down assay for S100A10 and annexin II complex. S100A10 and annexin II were analyzed in the lysate from membrane (m) and cytosol (c) fraction of TUHR14TKB and RCC10RGB9 cells (a) by western blotting or (b) after being pulled down with mAb S100A10–Sepharose beads. M indicates the marker for S100A10 and annexin II. (B) Immunofluorescent detection of (a) pre‐immune serum, (b) S100A10 and (c) annexin II on RCC10RGB9 cells. Cells were stained without being made permeable with NP‐40.