Figure 1.

 Overexpression of pescadillo in human primary breast cancer. (A) Western blotting analysis of pescadillo protein in normal breast epithelial cells (NBEC) and indicated breast cancer cell lines. α‐Tubulin was probed for loading control. (B) Expression of pescadillo mRNA in the NBEC and cultured breast cancer cell lines. Expression levels were normalized for GAPDH. (C) Western blotting analysis of pescadillo protein from human primary breast cancer (T) and paired tumor‐adjacent non‐cancerous breast tissues (n), with each pair taken from a same patient. (D) Real time RT‐PCR analysis of pescadillo mRNA from the same four pairs of breast cancer and adjacent non‐cancerous tissues. Error bars represent SDs calculated from three parallel experiments.