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. 2009 Aug 25;100(12):2255–2260. doi: 10.1111/j.1349-7006.2009.01325.x

Figure 3.

Figure 3

 Suppression of pescadillo inhibits breast cancer cell proliferation and tumorigenicity. (A) Western blotting analysis for pescadillo protein in MDA‐MB‐435 and ZR‐75‐30 cells transduced with RNAi vector control and pescadillo RNAi constructs (pescadillo‐RNAi‐1 and pescadillo‐RNAi‐2), respectively. (B) Growth curves of MDA‐MB‐435 and ZR‐75‐30 cells transduced with pescadillo RNAis as examined by MTT assay. Values presented represent means ± SD from three independent experiments. (C) The photos demonstrate results of colony‐formation assay of cells in the plate (left panel) and the mean (±SD) (right panel) colony numbers obtained from three independent experiments. (D) Silencing endogenous pescadillo abrogated the ability of anchorage‐independent growth of breast cancer cells. Colonies that contained more than 50 cells or were larger than 0.1 mm were scored (right panel). Each bar represents the mean ± SD of three independent experiments. *Statistical significance (P < 0.05).