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Effect of transfection with RET siRNA with Lipofectamine 2000 on Bcl‐2 expression and caspase 3/7 activity in TT cells. Cells were incubated for 72 h after transfection with 100 nm RET siRNA or Cont siRNA. Expressions of Bcl‐2 mRNA and protein were detected by quantitative RT‐PCR (A) and Western blot (B). Caspase 3/7 activity was measured by caspase 3/7 luminometric assay (C). n = 4 for each sample. **P < 0.01.
