Table 1.
Primers used to amplify regions of the human C1orf10 gene for DNA sequencing analysis
| Primer | Sequence (5′–3′) | Orientation | Ref. position | Amplification size | Region |
|---|---|---|---|---|---|
| P1 | CTTCAAACACCACCACAG | Forward | –2000 to –1983 | 782 | Promoter |
| P2 | TCTCCCTAGCAATCCTCT | Reverse | –1236 to –1219 | ||
| P3 | TTGGCTTCTGAGCTCAGAAAACTTC | Forward | –1485 to –1461 | 519 | Promoter |
| P4 | AGGCTTACCATGATCATGTGGTAAC | Reverse | –991 to –967 | ||
| P5 | TGCTGATTAGCCACCAGTTGAG | Forward | –1011 to –990 | 542 | Promoter |
| P6 | AGCAATTCCCTGCCTCAGACTC | Reverse | –490 to –469 | ||
| P7 | GGTCAGGAGTTCAAGATCAGC | Forward | –578 to –558 | 674 | Promoter 5′‐UTR Exon 1 |
| P8 | GCTCAGATCACCAAGACACTG | Reverse | +75 to +95 | ||
| P9 | GAAAGACCTAGCAGCAGACG | Forward | –97 (beginning of exon 2) | 361 | Exon 2 |
| P10 | AAATTCCCCAGGTCAAGGC | Reverse | +86 (beginning of intron 2) | ||
| P11 | GCTGCTGCTCTAGCCGTAT | Forward | –130 (beginning of exon 3) | 1100 | Exon 3 |
| P12 | CCTCCGTGGCTTACAGTTT | Reverse | +970 (beginning of exon 3) | ||
| P13 | AAGAACCAGCAAGCAGACAC | Forward | +633 (exon 3) | 778 | Exon 3 |
| P14 | TGCTCTTCAAAGGTAGGGAC | Reverse | +1800 (exon 3) |
Nucleotide positions are referenced relative to the major transcription start‐site of the human Chromosome 1 open reading frame 10 (C1orf10) sequence.