VHL gene dosage values detected by RQ‐PCR assay for the five primer/probe sets in 30 normal controls, 10 VHL deletion controls, and 33 Japanese patients. DNA samples of 30 healthy controls and patients, each of whom retained two copies of the VHL gene, displayed gene dosage values of approximately 1.0 (∼0.8 to ∼1.2), whereas the samples from 10 deletion controls and patients who each had a single copy of VHL displayed values of approximately 0.5 (∼0.4 to ∼0.6).