Figure 3.

 Cell mobility was strongly inhibited by Huaier extract (4 mg/mL) in invasive and migration assays. (a) Invasive assay of MDA‐MB‐231 cells using the Transwell system, which shows the inhibitory effect of 18 h of Huaier treatment on invasiveness. (b) The number of successfully invading MDA‐MB‐231 cells with or without Huaier treatment. (c) Migration assay of MDA‐MB‐231 cells after 5 h of treatment with Huaier. (d) The number of MDA‐MB‐231 cells, which successfully matrigel through membrane, was compared between control and Huaier‐treated group. (e) Migration assay of MCF‐7 cells after 5 h of treatment with Huaier. (f) The cell number decrease of the MCF‐7 cells indicates the great inhibitory effect of Huaier. Giemsa was used as a staining agent and cell numbers were counted at six representative fields. Data is presented as mean ± SD.