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. 2010 Jul 16;101(11):2375–2383. doi: 10.1111/j.1349-7006.2010.01680.x

Figure 6.

Figure 6

 Flow cytometric analysis of PI‐Annexin‐V to quantify Huaier‐induced apoptosis in MCF‐7 and MDA‐MB‐231 cells. (a) Dot plots of MDA‐MB‐231 cells with Huaier treatment at 0, 2 and 4 mg/mL for 24 or 48 h. Z‐VAD‐fmk was added to the culture medium 1 h before the treatment with 2 mg/mL 48 h Huaier extract. (b) Dot plots of MDA‐MB‐231 cells with Huaier treatment at 0, 4 and 8 mg/mL for 24 or 48 h. Z‐VAD‐fmk was added to the culture medium 1 h before the treatment with 4 mg/mL 48 h Huaier extract. The results shown are representative of three independent experiments.