Effects of Huaier extract on expression of p53, phosphorylated p53 (p‐p53), Bcl‐2, BCL2‐associated X protein (BAX) and caspase‐3 proteins. Expression of β‐actin was used as an internal control. (a,c) MCF‐7 cells and (b,d) MDA‐MB‐231 cells were treated with 4 mg/mL for 24 or 48 h and 8 mg/mL for 24 or 48 h. The expression of each protein was quantified as the densitometry value analyzed by ImageJ. (e) Different expression of Bcl‐2 in MCF‐7 and MDA‐MB‐231 cells. The figure shown is representative of three independent experiments.