Figure 2.

α‐Galactosylceramide (α‐GalCer)‐induced interferon (IFN)‐γ was not detected in natural killer (NK) T cells in interleukin (IL)‐4^−/– mice even at single‐cell level. The IFN‐γ‐ and IL‐4‐producing abilities of hepatic NK1.1⁺ CD3⁺ NKT cells from wild‐type (wt) or IL‐4^−/– mice were examined by flow cytometry (a,d) 1, (b,e) 2 and (c,f) 4 h after intravenous administration of 2 µg α‐GalCer. The experiment shown is representative of three independent experiments.