Table 1.
Spleen cells were prepared from wild‐type or interleukin (IL)‐4−/– mice treated with α‐galactosylceramide (α‐GalCer) (2 µg/head) or saline 2 h before death
| Mouse | α‐GalCer | IFN‐γ (pg/mL) | ||||
|---|---|---|---|---|---|---|
| In vivo (2 µg/head) | In vitro (200 ng/mL) | 4 h | 24 h | 48 h | 72 h | |
| Wild type | – | – | – | ND | ND | ND |
| – | + | – | 785 ± 9 | >2000 | >2000 | |
| + | – | 299 ± 15 | >2000 | >2000 | >2000 | |
| + | + | – | >2000 | >2000 | >2000 | |
| IL‐4−/– | – | – | – | ND | ND | ND |
| – | + | – | ND | ND | >2000 | |
| + | – | ND | ND | ND | ND | |
| + | + | – | ND | ND | >2000 | |
The cells were cultured with or without α‐GalCer (200 ng/mL) for 4, 24, 48 or 72 h. Interferon (IFN)‐γ levels in supernatants were decided by enzyme‐linked immunosorbent assay. The values represent mean ± SE of duplicated samples. The experiment shown is representative of three independent experiments ND, not detectable.