Figure 2.

Detection of transient receptor potential melastatin 7 (TRPM7) mRNA and protein in human gastric adenocarcinoma cell lines. (a) Reverse transcrption–polymerase chain reaction detection of TRPM7 mRNA in human gastric adenocarcinoma cell lines. HEK293 cells with inducible expression of TRPM7 served as a positive controls and β‐actin was used as an internal control. (b) TRPM7 proteins were detected at molecular weight 220 kDa by western blotting. HEK293 cells with inducible expression of TRPM7 served as a positive control. (c) TRPM7 immunoreactivity in AGS cell under confocal microscopy (left) and its differential interference contrast image (right). Horizontal bar indicates 10 µm.