Figure 4.

MGC cells were stimulated with recombinant IL‐1β 10 ng/mL. Phosphorylation status of pIκB, pErk1/2 and pAKT was confirmed with western blotting. Anti‐α Tubulin was treated as internal control. Maximal phosphorylation of IκB was seen 5 min after stimulation of IL‐1β, then gradually decreased, but was re‐up‐regulated 45 min after stimulation. Maximal phosphorylation of Erk1/2 was observed at 10 and 15 min, and the level gradually decreased with time. By comparison, the increased phosphorylation of Akt was seen at 5 and 10 min, and was still elevated at the endpoint.