Figure 5.

NF‐κB2/p58 activates the endogenous NF‐κB2/p100 expression in a T‐cell line. (a, b) The cell lysates were prepared from TL‐OmI (lane 1), and CTLL‐2 infected with either p58‐virus (lane 2), p52‐virus (lane 3), control virus (lane 4) or Tax‐virus (lane 5). NF‐κB2 proteins in cell lysates were analyzed by a Western blot analysis using an anti‐p100 antibody. The CTLL‐2 cells expressing p58 or p52 in (a) and (b) were independently established. (c) The CTLL‐2 cells characterized above were cultured in the absence of IL‐2, and viable cell numbers were counted using the trypan blue exclusion assay under microscopy.