Figure 3.

 Induction of tumor‐specific CTL by intracellular hyperthermia. Analysis of spelenocytes cytotoxic activity against B16‐OVA melanoma cells, EL4, EL4 pulsed with SL8 peptide, and YAC‐1 in ⁵¹Cr release assay. Mice were treated by N‐propionyl‐4‐S‐cysteaminylphenol with magnetite nanoparticles (NPrCAP/M) injection followed by alternating magnetic field (AMF) exposure. Four weeks after treatment, spleen cells were removed and stimulated with irradiated B16‐OVA cells. Cytotoxic activity of spleen cells against B16‐OVA cells, EL4 cells, EL4 cells pulsed with SL8 peptide, or YAC‐1 cells was determined by standard ⁵¹Cr‐release assay. Bars, SD.