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. 2010 May 19;101(9):1939–1946. doi: 10.1111/j.1349-7006.2010.01623.x

Figure 4.

Figure 4

 Heat shock protein (HSP) expression in tumor cells after intracellular hyperthermia. (a–c) B16‐OVA cells were subjected to hyperthermia using N‐propionyl‐4‐S‐cysteaminylphenol with magnetite nanoparticles (NPrCAP/M) with alternating mag‐netic field (AMF) exposure in vitro. Seventy‐two hours after intracellular hyperthermia (+) or left untreated (–), the expression of Hsp72 and Hsp90 was determined by western blotting with an anti‐Hsp72/Hsc73 mAb or anti‐Hsp90 mAb (a). *Hsc73, **Hsp72, ***Hsp90. The expression of Hsp72 was markedly enhanced by intracellular hyperthermia. Heat shock protein (HSP) in the lysate was quantified by the Hsp72 ELISA (b) and Hsp90 ELISA kits (c). (d) Hsp72 and Hsp90 in the 12‐, 24‐ and 48‐h culture supernatant after intracellular hyperthermia were measured using ELISA. Bars, SD. Results shown are representative of three different experiments.