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. 2010 May 19;101(9):1939–1946. doi: 10.1111/j.1349-7006.2010.01623.x

Figure 5.

Figure 5

 CD8+ T‐cell response against dendritic cells (DCs) pulsed with B16‐OVA melanoma cell lysate after intracellular hyperthermia and effect of heat shock protein (HSP) depletion. (a) B16‐OVA cells were subjected to N‐propionyl‐4‐S‐cysteaminylphenol with magnetite nanoparticles (NPrCAP/M) exposure and alternating magnetic field (AMF) irradiation, then lysed by freezing and thawing. Dendritic cells (DCs) were pulsed with the cell lysate, and cultured overnight with B3Z and the absorbance of β‐galactosidase activity was measured at 595 nm. Bars, SD. (b) Lysates of the cells were immunoprecipitated with anti‐Hsp72/Hsc73 antibody, anti‐Hsp90 antibody and anti‐KDEL antibody. Immunoprecipitates were removed from lysate. After being pulsed with the HSP‐depleted lysate, DCs were incubated with B3Z T cell hybridoma and the absorbance of β ‐galactosidase activity was measured at 595 nm. Bars, SD. (c) Cell lysates were depleted of HSPs (above lanes) with antibodies specific for each HSP. Immunoblots were of depleted (D) or undepleted (UD) lysates. Results shown are representative of five different experiments.