Figure 1.
Conditional expression of KrasG12V transgene. (A) The CALNLHAKrasG12V transgene is comprised of a hybrid CMV enhancer/chicken β‐actin (CAG) promoter, a cassette for the neomycin resistance gene flanked by loxP sites, and a sequence containing a human KrasG12V with a hemagglutinin (HA)‐tag. Infection with the Cre recombinase‐expressing adenovirus results in Cre‐mediated recombination of the transgene and removal of the neo‐coding region and its associated mRNA polyadenylation signal, generating a functional HA‐KrasG12V gene expression unit. GpA, rabbit‐ β‐globin poly(A) site; pA, SV40 early poly(A) site. (B) Cre recombinase with nuclear localization signal expressing adenovirus in which Cre expression is under the control of three different promoters: the amylase promoter and the elastase‐1 promoter which are active in acinar cells, and the CAG promoter which is a nonspecific promoter.