Figure 2.

 Acinar cell‐specific expression of hemagglutinin (HA)‐Kras^G12V. (A) Localization of amylase (blue) protein, HA‐Kras^G12V (red) and Ki67 (green) at 2 days after injection of virus with Ad‐Amy‐Cre (a, b, c, d) and Ela‐Cre (e). All the HA‐Kras^G12V positive cells (red) were acinar cells; expression was not observed in duct cells (**), centroacinar cells (yellow arrowhead), or small duct cells (*). Most virally infected acinar cells positive for HA‐Kras^G12V were indistinguishable from non‐infected acinar cells by hematoxylin–eosin staining. Some infected acinar cells have nuclei with a so‐called “owl‐eye” (yellow arrows) or “ground glass” (red arrows) appearance. Ki67 (green) is not present in the nuclei of the cells expressing HA‐Kras^G12V (red). Bar, 20 μm. (B) None of the Ad‐Amy‐Cre (a) or the Ad‐Ela‐Cre (b) groups displayed any pancreatic lesions, even after 8 weeks. Bar, 50 μm.