Figure 4.
Pancreatic ductal adenocarcinoma (PDA) induced by injection of Ad‐CAG‐Cre in Kras301/327 rats. (A) The expression of hemagglutinin (HA)‐KrasG12V (red) was seen only in PDA lesions (on the left of photo), and not in stromal cells, acinar cells (on the right of photo), or normal pancreatic duct cells (*). Bar, 500 μm. (B) A pancreatic intraepithelial neoplasia (PanIN) lesion was surrounded by fibrous tissue with some infiltration of inflammatory cells. Expression of proliferating cell nuclear antigen (PCNA) (green) and HA protein (red) in a PanIN lesion in rats of the CAG‐Cre group. PCNA is preferentially expressed in PanIN cells. Bar, 20 μm. (C) Expression of Ki67 (green) and HA protein (red) in PDA cells. Many PDA cells (red) are simultaneously positive for Ki67. Bar, 50 μm.