Figure 1.

Identification of the melanoma inhibitory activity (MIA) binding partner cadherin‐7. (A) Surface‐enhanced laser desorption/ionization (SELDI) technology revealed specific binding of MIA and cadherin‐7. Co‐immunoprecipitation (CoIP) was performed using protein lysates of Mel Im incubated with biotinylated MIA or TANGO. Coomassie staining showed additional bands through interaction with MIA in comparison to the negative control and TANGO. SELDI mass spectrometry followed by digestion and analyses of gel bands identified one band as cadherin‐7. (B) Co‐immunoprecipitations confirmed the direct interaction of MIA and cadherin‐7. For immunoprecipitation an anti‐cadherin‐7 antibody (a‐Cad7) was used. Interaction was detected in three melanoma cell lines using western blot analysis with MIA antibody. Recombinant MIA was used as positive control.