Figure 3.

Role of cadherin‐7 in the migration of melanoma cells derived from primary tumors. (A) The primary melanoma cell line Mel Juso was stably transfected with an antisense cadherin‐7 construct and cell clones (asCad7 #4 and asCad7 #6) were analyzed by fluorescence‐activated cell sorting analysis. The gray lines present anti cadherin‐7 antibody staining. The black lines mark the negative controls. AsCad7 clones showed a reduced expression of Cad7 in comparison to the control‐transfected mock clones (Mock 1, Mock 3). (B) The Boyden chamber assay revealed a reduced migration of the asCadherin‐7 transfected Mel Juso cells compared to the mock control. (C) Compared with control cells, Mel Juso cells transiently transfected with a cadherin‐7 expressing vector showed a decreased migration capability.