Figure 4.

Role of cadherin‐7 in the migration of melanoma cells derived from metastases. (A) Mel Im cells were stably transfected with a cadherin‐7 overexpressing vector and cadherin‐7 expression was analyzed by fluorescence‐activated cell sorting analysis. The cCad7 cell clones (cCad7 #1, #3) showed induced cadherin‐7 expression compared to the control‐transfected mock clone and the parental Mel Im cell line. (B) The cCad7 cell clones showed a decreased migration in comparison to the control in the Boyden chamber assay. (C) The Boyden chamber assay revealed a reduced migration after treatment of the mock control cells with recombinant melanoma inhibitory activity (MIA) (200 ng/mL). In contrast, in cadherin‐7 overexpressing cells migration stayed unchanged after treatment with recombinant MIA.