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. 2010 Mar 4;101(6):1511–1520. doi: 10.1111/j.1349-7006.2010.01551.x

Figure 1.

Figure 1

 (A) Schematic map of 3p22.3 region with microsatellite and sequence tag site (STS) markers used in this analysis: dark lines (D1, D2) indicate deleted regions identified in our study. (B) Autoradiograph showing loss of heterozygosity (LOH), microsatellite size alteration of one allele (MA‐I), and loss of one allele and size alteration of the other (LOH + MA) (a). ↑ indicates loss of corresponding alleles and indicates size alteration of one or both alleles. Homozygous/hemizygous deletion (HD\HED) of RB1 serine phosphates from human chromosome 3 (RBSP3) and integrin α RLC (ITGA9) loci (b). (C) Representative autoradiographs of Southern hybridization showing homozygous (HD) and hemizygous (HED) deletions of RBSP3 and ITGA9 loci. CD3γ was used as a control locus. Marker, λHindIII marker; ↑, allelic loss. (D) Histogram showing genetic alterations of the 3p22.3 region in head and neck squamous cell carcinoma (HNSCC) (a) and dysplastic lesions (b); D1 and D2 are highly deleted regions. N, DNA from normal tissue/peripheral blood lymphocyte (PBL); T, tumor DNA.