Figure 4.
Effect of Hakai overexpression on the proliferation and migration of breast cancer cells. (a) Hakai overexpression inhibits the estrogen‐induced proliferation of Mcf‐7/Hakai stable cells. Cells were grown in the absence or presence of 300 ng/mL doxycycline and 100 nM estradiol. Cell proliferation was determined by the incorporation of [3H]thymidine during the last 4 h of culture. Data are representative of at least three independent experiments. Doxycycline‐dependent expression of Hakai in Mcf‐7/Hakai cells was examined by western blotting analysis. NS, non‐specific. **P < 0.005, significant differences from the control. (b) Hakai overexpression inhibits the estrogen‐induced migration of Mcf‐7/Hakai cells in wound‐healing assays. Uniform scratches created on the confluent cultures were sealed by culturing for 6 h in the presence or absence of 100 nM estradiol.