Figure 2.

 Co‐activation of multiple receptor tyrosine kinases (RTKs) in a primary malignant pleural mesothelioma (MPM) specimen. (a) A phospho‐RTK array (R&D Systems, Minneapolis, MN, USA) was performed using protein lysate extracted from surgically resected human MPM specimen (MMY‐39). Positive duplicated spots for individual RTK were numbered and indicated below the array. Five of 42 RTKs were scored positive. Two dots at each corner indicate positive controls. (b) Expression of MET and epidermal growth factor receptor (EGFR) and their phosphorylation (phospho‐MET and phospho‐EGFR) were detected with immunohistochemical analysis. DTK, developmental tyrosine kinase; MSP‐R, macrophage‐stimulating protein receptor; M‐CSF‐R, macrophage‐colony stimulating factor receptor.