FIG. 1.

(A) Replication reactions performed with mixtures of Ad-infected and uninfected extracts. Band A is full-length duplex AAV DNA; band AF is a faster-migrating form of full-length AAV DNA. (Band AF is a closely spaced doublet.) (B) Assays performed with extracts, unsupplemented or supplemented with single-stranded DNA binding proteins. Lanes: 1, unsupplemented Ad-infected extract; 2, unsupplemented extract from uninfected heat-shocked cells; 3, uninfected extract supplemented with the Ad-DBP; 4, uninfected extract supplemented with E. coli SSB; 5, uninfected extract supplemented with RPA; 6 uninfected, unsupplemented extract.