FIG. 3.

(A) Replication in Ad-infected extracts in which aliquots were withdrawn at the times shown (in hours). (B) Pulse-chase experiment with Ad-infected extracts. At 2.5 h after the addition of Rep, the reaction mixture was given a pulse of [³²P]dCTP, followed 20 min later by 300-fold chase of cold dCTP. Aliquots were withdrawn after the chase at the times indicated above each lane. Single-stranded/double-stranded DNA ratios (as determined by PhosphorImager analysis) are given below each lane. (C) Pulse-chase experiment with uninfected extract. At 2.5 h after the addition of Rep, the reaction mixtures were given a pulse of [³²P]dCTP, followed 20 min later by a 300-fold chase of cold dCTP. Aliquots were withdrawn at the indicated times after the chase.