FIG. 6.

Camptothecin inhibition of replication in Ad-infected extracts of a one-ITR substrate. A designates duplex full-length AAV; V designates linear, duplex pBR; AX designates duplex AAV digested with XhoI; and AF designates single-stranded AAV DNA. Shown above each lane are the addition of R (Rep 68) and/or C (camptothecin) at either the 0.0- or the 2.5-h time point. Radioactive label was added just after the 2.5-h time point; the reactions were allowed to proceed for an additional 13.5 h. Shown below is a diagram of the substrates.