FIG. 8.

Two-dimensional gel analysis of the products of an assay performed with uninfected extracts supplemented with RPA. The diagram denotes the origin of species seen on the gel, as described in the text. HA and A are full-length duplex hairpinned and nonhairpinned AAV, respectively. AF is full-length single-stranded AAV. H-arc is a collection of AAV molecules with an ITR in the closed-hairpin conformation containing one complete AAV strand and various lengths of the second strand. N-arc is a collection of incomplete second strands, which are derived from molecules with an ITR which has been nicked and which contained one complete AAV strand and various lengths of the second strand.