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. 2010 Jan 20;101(5):1177–1185. doi: 10.1111/j.1349-7006.2010.01503.x

Figure 6.

Figure 6

 Non‐acetylation‐mimicking Foxo3a overexpression suppresses cancer cell growth and cisplatin resistance to a lesser extent compared with wild‐type Foxo3a overexpression. (a) Whole‐cell extracts of the indicated stable transfectants of KK47 cells were subjected to SDS‐PAGE, and western blotting was performed using the indicated antibodies. Immunoblot for β‐actin is shown as a loading control. (b) Whole‐cell extracts (500 μg) of the indicated stable transfectants of KK47 cells were immunoprecipitated with antibody against GFP and A/G agarose. After washing three times, the supernatants were subjected to SDS‐PAGE, and western blotting was performed using the indicated antibodies. (c) The indicated stable transfectants of KK47 cells were seeded into 12‐well plates and incubated. The number of cells was counted at the indicated times. The results were normalized to the number of cells at 0 h. All values represent at least three independent experiments. Boxes, mean; bars, ±SD. (d) The indicated stable transfectants of KK47 cells were seeded into 96‐well plates. The following day, various concentrations of cisplatin were applied. After incubation for 48 h, cell survival was analyzed by a cytotoxicity assay. Cell survival in the absence of cisplatin corresponds to 1. All values are representative of at least three independent experiments. Boxes, mean; bars, ±SD.