Table 1.

 Application of PCR

Gene	Primer sequences	Number of cycles	Annealing temperature	Product size (bp)
VEGF‐D	F: 5′‐GTATGGACTCTCGCTCAGCAT‐3′	32	59	226
	R: 5′‐AGGCTCTCTTCATTGCAACAG‐3′
VEGFR‐2	F: 5′‐GCATCTCATCTGTTACAGC‐3′	32	62	331
	R: 5′‐CTTCATCAATCTTTACCCC‐3′
VEGFR‐3	F: 5′‐GGTTCCTCCAGGATGAAGAC‐3′	32	62	505
	R: 5′‐CAAGCAGTAACGCCAGTGTC‐3′
β‐Actin	F: 5′‐GGACTTCGAGCAAGAGATGG‐3′	35	55	234
	R: 5′‐AGCACTGTGTTGGCGTACAG‐3′
GAPDH	F: 5′‐ATCATCCCTGCCTCTACTGG‐3′	28	55	188
	R: 5′‐CCCTCCGACGCCTGCTTCAC‐3′
Bcl‐2	F: 5′‐GGTGGAGGAGCTCTTCCAGG‐3′	35	60	206
	R: 5′‐ACAGTTCCACAAAGGCATCC‐3′

VEGF‐D, vascular endothelial growth factor D; VEGFR‐2, VEGF receptor 2.