Figure 1.

 Study design of the gene expression analysis. Cryopreserved PBMC samples of pre‐ and post‐αGalCer‐pulsed DC therapy were thawed. CD56⁺ cells and CD56⁻CD3⁺ T cells were purified using magnetic beads and an Auto‐MACS sorter. mRNA was extracted from CD56⁺ cells and CD56⁻CD3⁺ T cells and a microarray analysis was performed in six patients. Three patients were from the good responder group and three were from the poor responder group. The candidate genes identified by the microarray data were validated with quantitative RT‐PCR (qRT‐PCR).