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(a) Expression of Myc‐tagged IGSF11 in NIH3T3 cells transfected with pcDNA3.1myc/His‐IGSF11 was examined by Western blot analysis using anti‐Myc antibody. (b) Colony formation activity of exogenous IGSF11 in NIH3T3 cells. (c) Gene‐knockdown effect of IGSF11‐specific siRNA. (d) Significant growth‐inhibitory effect of the IGSF11‐specific siRNA in St‐4 cells (P < 0.05).
