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. 2009 Nov 23;101(3):700–706. doi: 10.1111/j.1349-7006.2009.01448.x

Figure 3.

Figure 3

 Wnt‐3a increased the DIX Domain Containing 1 (DIXDC1) protein level, but not the DIXDC1 mRNA expression in vitro. (a) HeLa cells were cultured in Minimum Essential Media with or without Wnt‐3a (80 ng/mL) for the indicated time periods. β‐Catenin and DIXDC1 proteins were examined by western blot analysis. Protein levels were normalized to β‐actin. (b) DIXDC1 mRNA levels in Wnt‐3a‐treated HeLa cells at different time intervals were evaluated by real‐time reverse transcription–polymerase chain reaction assay. Only weak mRNA expression stimulation was observed at 4 and 8 h after Wnt‐3a treatment. DIXDC1 mRNA expression decreased after 12‐h treatment with Wnt‐3a. Consistent results were obtained in at least three independent experiments. Data represent the mean ± SD of three independent experiments. Least significant difference t‐test was used to identify significant differences in multiple comparisons. *P < 0.01 compared with 0 h; **P < 0.01 compared with 4 h, ***P < 0.01 compared with 8 h, and ****P < 0.05 compared with 12 h.