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. 2006 Dec 13;98(2):189–200. doi: 10.1111/j.1349-7006.2006.00375.x

Figure 2.

Figure 2

Construction and analysis of ts‐FL‐p53, ts‐Δ1stTAD and ts‐TAD‐S/A. (A) The constructs used in this study are shown. In addition to the ts mutation at codon 138, Δ1stTAD lacks the first 39 amino acids of FL‐p53 and TAD‐S/A carries mutations at all serine residues within the first and the second TAD (Ser 6, 9, 15, 20, 33, 37 and 46 were converted to Ala). Each construct was cloned in pMX‐puro retroviral vector, and is C‐terminally tagged simultaneously with FLAG, HA, and polyhistidine peptide (shown as FHH). TAD, transactivation domain; P, proline‐rich domain; DBD, DNA binding domain. (B) The luciferase reporter assay was performed using the above constructs. The assay was performed using Saos2 cells at non‐permissive (38°C, shown as white bars) and permissive (32°C, shown as gray bars) temperatures.