Figure 3.

Selective replication of Ad5WS2 in human and murine hepatocellular carcinoma (HCC) cell lines. (a) Ad5WS2 induced more cell death in human and murine HCC cells, but was highly attenuated in non‐HCC cells. Cells were infected with Ad5WS2 or Ad5WS1 at an multiplicity of infection (MOI) of 2. The viable cell numbers were determined after 3 days by trypan blue exclusion. Each value represents the mean ± SD (n = 3). *P < 0.05; ***P < 0.001.(b) Human and murine HCC cells produced higher viral titers than cancer cells from non‐liver origin following infection with Ad5WS2. Various cells were infected with Ad5WS2 or Ad5WT at an MOI of 10. Fifty‐three hours post‐infection, viral titers were determined in 293 cells by the TCID₅₀ method. The amount of Ad5WS2 produced was normalized against the amount of Ad5WT produced in the same cell line. Relative viral production was indicated by comparing each cell line with 293 cells. (c) Expression of adenoviral E1A protein was detected in Hep3B and ML‐1 cells at 36 h post‐infection with Ad5WS2 at an MOI of 10. (d) Expressions of adenoviral hexon and fiber proteins were detected in Hep3B and ML‐1 cells at 48 h post‐infection with Ad5WS2 at an MOI of 1.