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. 2007 Feb 12;98(4):563–568. doi: 10.1111/j.1349-7006.2007.00426.x

Figure 3.

Figure 3

Effect of tissue inhibitor of metalloproteinase‐2 (TIMP‐2) on matrix metalloproteinase (MMP)‐2 activation by membrane‐type (MT) 1‐MMP. (a) Control plasmid or expression plasmid for MT1‐MMP (100 ng) was cotransfected with the indicated amounts of TIMP‐2 plasmid into 293T cells cultured in 48‐well plates in duplicate. At 48 h after transfection, cells were incubated with MMP‐2 for 1 h. Then, supernatants and cells were analyzed by gelatin zymography (upper panel) and for gelatin‐degradation activity (lower panel) as described in ‘Materials and Methods’. (b) 293T cells transfected with control plasmid or expression plasmids for MT1‐MMP (100 ng) and TIMP‐2 (25 ng) were incubated with MMP‐2, and gelatin degradation was examined as above in the presence or absence of 1 µg/mL TIMP‐1 or TIMP‐2. (c) 293T cells were transfected with control plasmid or expression plasmids for MT1‐MMP (100 ng) and TIMP‐2 (25 ng) as indicated, and were incubated with labeled gelatin for 1 h at 48 h after transfection.