Figure 4.

Effect of tissue inhibitor of metalloproteinase‐2 (TIMP‐2) on testican‐1 cleavage by membrane‐type (MT) 1‐matrix metalloproteinase (MMP). Testican‐1‐FLAG sample was incubated with 293T cells mock‐transfected or cotransfected with MT1‐MMP (700 ng) and indicated amounts of TIMP‐2 plasmid for 3 h. Cleavage of testican‐1‐FLAG was analyzed by western blotting using anti‐FLAG M2 antibody (left panel). Testican‐1‐FLAG sample was incubated with cells mock‐transfected or transfected with MT1‐MMP plasmid in the presence or absence of TIMP‐1 or TIMP‐2 (1 µg/mL) for 3 h, and was analyzed as above (right panel).