Table 1.

 Sequences and positions of primers used for amplification and sequencing of the Enh1/X‐promoter regions, cloning, and measurement of X‐gene mRNA

Position	Nucleotide sequence (5′–3′)	Polarity
Primer for PCR and sequencing
For 1st round of PCR
953→968	AAC TKC CTG TAA AYC AG	Sense
1433→1416	GGG ACG TAA RAC AAA GGA C	Antisense
For 2nd round of PCR
970→988	CCT ATT GAT TGG AAA GTW TG	Sense
1430→1413	ACG TAR ACA AAG GAC GTC	Antisense
Primer for cloning (pGL3 basic)
950→966	CTA GCT AGC GGA AAC TGC CTG TAA AT (including the restriction enzyme NheI site)	Sense
1373→1354	GGT GCA AGC TTG GGA AGG AGG TGT ATT TCC G (including the restriction enzyme HindIII site)	Antisense
Primer for mRNA of X‐gene
1374→1400	ATG GCT GCT CGG GTG TGC TGC CAA CTG	Sense
1602→1579	GTG CAG AGG TGA AGC GAA GTG CAC	Antisense