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. 2010 Nov 16;101(12):2531–2537. doi: 10.1111/j.1349-7006.2010.01702.x

Figure 1.

Figure 1

 Production of excess centrosomes in human U2OS cells and mouse NIH3T3 cells after acute and protracted exposures to γ‐irradiation. (a) U2OS cells were irradiated with 5 Gy at a dose rate of 1 Gy/min (left panel), and with a total dose of 5 Gy at a dose rate of 0.5 mGy/min (right panel). Cells were examined for excess centrosomes at the indicated time points using γ‐tubulin antibodies. (b) Dose–response curves for centrosome overduplication. U2OS (left panel) and NIH3T3 (right panel) cells were irradiated with the indicated doses at a dose rate of 1 Gy/min and 0.5 mGy/min, and centrosome numbers were counted 96 h after irradiation. (c) Cell survival after protracted exposures to ionizing radiation. U2OS (left panel) and NIH3T3 (right panel) cells were irradiated with the indicated doses at a dose rate of 1 Gy/min and 0.5 mGy/min. (d) Roscovitine, a cyclin‐dependent kinase inhibitor, had no effect on the survival of NIH3T3 cells after exposure to 5 Gy of ionizing radition, but strongly inhibited the induction of centrosome overduplication. Bars represent the standard errors (n = 3).