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. 2010 Nov 16;101(12):2531–2537. doi: 10.1111/j.1349-7006.2010.01702.x

Figure 3.

Figure 3

 Centrosome overduplication in homologous recombination repair protein‐deficient cells. (a) Brca1−/− and Brca1+/− embryonic stem (ES) cells were stained with anti‐γ‐tubulin and anti‐centrin‐2 antibodies after irradiation with 5 Gy at dose rates of 1 Gy/min and 0.5 mGy/min. Cells were irradiated with the indicated doses and centrosomes were quantified 72 h after irradiation. (b) As in (a), Nbs1−/− fibroblast cells (A31‐1) and complemented A31‐1 + NBS1 cDNA cells were stained, and centrosomes were quantified 72 h after irradiation. Bars represent the standard errors (n = 3).