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. 2008 Oct 23;99(11):2268–2273. doi: 10.1111/j.1349-7006.2008.00939.x

Figure 3.

Figure 3

Cytotoxic T lymphocytes (CTL) were induced by dendritic cells (DC) pulsed with idiotype–monosodium urate (Id‐MSU) complex. Effector cells were stimulated by DC for 2 weeks. Cytotoxicity was measured by lactate dehydrogenase release assay. (a) Cytotoxicity of effector cells from human leukocyte antigen (HLA)‐A2‐positive volunteers against target cells generated by DC pulsed with IM‐9 Id‐MSU complex was compared to control antigens; control Id‐MSU complex, MSU crystals alone, Id alone, or nothing. *P < 0.01 (vs all other controls). (b) Cytotoxicity of effector cells educated by IM‐9 Id‐MSU complex‐pulsed DC was compared to those generated by MSU bathed in 10% fetal bovine serum solution for 3 h before mixing with Id to block MSU binding with Id. *P < 0.01. (C) Cytotoxicity of effector cells from HLA‐A2‐positive volunteers was compared to that of HLA‐A2‐negative volunteers against target cells. *P < 0.01. Data are expressed as mean ± standard deviation of three independent experiments.