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Figure 3.

Figure 3

In vitro invasion assay of the human colon cancer cell line, SNU‐81 after suppression of the ATP synthase α‐ and d‐subunit. SNU‐81 cells were transfected with either small interfering RNA (siRNA) specific for ATP synthase α‐ and d‐subunit, non‐silencing control siRNA, or phosphate‐buffered saline (PBS) buffer only. (a) Expression of the ATP synthase α‐ and d‐subunits was efficiently suppressed at 96 h after transfection with ATP synthase d‐subunit siRNA. (b) At 9 h after transfection, ATP synthase α‐ and d‐subunit siRNA‐transfected cells showed decreased invasiveness compared with those transfected with either non‐silencing control siRNA or PBS.