FIG. 2.

Association of E4orf6 but not E4orf6Δ225-232 with E1B-55 kDa. E4orf6 and its mutant E4orf6Δ225-232 were synthesized and radioactively labeled by transcription and translation in vitro (lanes 1 and 2). The proteins were then incubated with lysates from HeLa cells (lanes 3 and 4) or 293 cells (containing E1B-55 kDa; lanes 5 and 6). After immunoprecipitation with a monoclonal antibody (2A6) against E1B-55 kDa, the precipitated material was separated by SDS-polyacrylamide gel electrophoresis and exposed to a BioImager screen. wt, wild type.