FIG. 6.

Replication of viral DNA upon E4orf6 expression. (A) HeLa cells were electroporated and infected as described in the legend to Fig. 3. At the time points indicated after infection, DNA was isolated, treated with HindIII, separated by agarose electrophoresis, and blotted. A genomic fragment corresponding to the adenovirus E1B region was detected by Southern hybridization. GFP, green fluorescent protein; wt, wild type. (B) The cells were transfected (Superfect) with wild-type E4orf6 along with expression plasmids for the export competitor as indicated. Virus DNA was detected by Southern blotting.