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Detection of osteopontin (OPN) and caspase‐3 in human hepatocellular carcinoma (HCC) cell lines by western blot analysis (a). Relative quantitation is also shown. OPN expression increased with increasing metastatic potential in human HCC cell lines (b). Caspase‐3 decreased with increasing metastatic potential (c). A monoclonal antibody against β‐actin was used as an internal control.
