Skip to main content
. 2009 Jul 30;100(11):2218–2225. doi: 10.1111/j.1349-7006.2009.01301.x

Figure 5.

Figure 5

 Bone morphogenetic protein (BMP) 7 inhibited melanoma cell migration ability and reduced resistance to cisplatin. (A) WM‐266‐4 cells were treated with 100 ng/mL BMP7 or 4 μmol/L cisplatin in 0.1% serum medium for 8 days and grown to 90% confluency. A wound line was made on the monolayer cultures with a microtip, cells were washed twice with PBS, and fresh medium was added. Photographs were taken at 12‐h time points. Migrating cell counts were analyzed using Image Pro Software. BMP7 reduced cell migration ability to <50% of that in the control, whereas cisplatin only slightly inhibited migration ability. Bars, SD. **P < 0.0074; ***P < 0.0006. (B) WM‐266‐4 cells were treated with BMP7 as indicated for 3 days, then with cisplatin at various concentrations for an additional 8 days. Cell resistance to cisplatin gradually decreased with increasing BMP7 concentrations. Bars, SD; *P < 0.005.