Table 1.

 Primer sequences and product sizes for RT‐PCR

Target	Primer sequence	Product size base pairs	Melting temperature (°C)	Amplification cycles
Alk2	5′‐GCATTCCCAGAGCACCAATC	383	54	30
	3′‐CTGTGAGTCTTGCGGATGGA
Alk6	5′‐GCAGCACAGACGGATATTGT	630	50	30
	3′‐TTTCATGCCTCATCAACACT
noggin	5′‐CACTACGACCCAGGATTCAT	213	55	25
	3′‐CTCCGCAGCTTCTTGCTTAG
SMA	5′‐AGGAAGGACCTCTATGCTAACAAT	355	54	25
	3′‐AACACATAGGTAACGAGTCAGAGC
Slug	5′‐ACGCCTCCAAAAAGCCAAAC	517	58	30
	3′‐GGTAATGTGTGGGTCCGAAT
TWIST	5′‐GGAGTCCGCAGTCTTACGAG	201	56	30
	3′‐TCTGGAGGACCTGGTAGAGG
snail	5′‐CCTCCCTGTCAGATGAGGAC	234	57	30
	3′‐CCAGGCTGAGGTATTCCTTG
GAPDH	5′‐TCTAGACGGCAGGTCAGGTCCACC	598	58	25
	3′‐CCACCCATGGCAAATTCCATGGCA